ऋषियों द्वारा प्रदत्त ‘आयुर्वेद’ के वैज्ञानिक अनुसंधान के पूरक, पतंजलि रिसर्च इंस्टीट्यूट (PRI) का एक और प्रमाणिक अन्वेषण! पुत्रजीवन (Piutranjiva roxburgi Wall) बीर्जों Zebrafish ds Infertility model में अनूठा एवं सफल प्रयोग…. Male और Female दो

ऋषियों द्वारा प्रदत्त ‘आयुर्वेद’ के वैज्ञानिक अनुसंधान के पूरक, पतंजलि रिसर्च इंस्टीट्यूट (PRI) का एक और प्रमाणिक अन्वेषण! पुत्रजीवन (Piutranjiva roxburgi Wall) बीर्जों Zebrafish ds Infertility model में अनूठा एवं सफल प्रयोग…. Male और Female दोनों Infertility conditions में पुत्रजीवक के बीजों का असरदायक प्रभाव, ‘‘Molecules’’ जर्नल के प्रकाषित… जय अयुर्वेद! जय विज्ञान! जय पतंजलि! अधिक जानकारी के लिए नीचे दिये गये लिंक पर जाएं- https://doi.org/10.3390/molecules26041020 Supercritical Fluid Extract of Putranjiva roxburghii Wall. Seeds Mitigates Fertility Impairment in a Zebrafish Model by Acharya Balkrishna 1,2, Pradeep Nain 1, Monali Joshi…

ऋषियों द्वारा प्रदत्त ‘आयुर्वेद’ के वैज्ञानिक अनुसंधान के पूरक, पतंजलि रिसर्च इंस्टीट्यूट (PRI) का एक और प्रमाणिक अन्वेषण! पुत्रजीवन (Piutranjiva roxburgi Wall) बीर्जों Zebrafish ds Infertility model में अनूठा एवं सफल प्रयोग…. Male और Female दोनों Infertility conditions में पुत्रजीवक के बीजों का असरदायक प्रभाव, ‘‘Molecules’’ जर्नल के प्रकाषित… जय अयुर्वेद! जय विज्ञान! जय पतंजलि! अधिक जानकारी के लिए नीचे दिये गये लिंक पर जाएं- https://doi.org/10.3390/molecules26041020

Supercritical Fluid Extract of Putranjiva roxburghii Wall. Seeds Mitigates Fertility Impairment in a Zebrafish Model

by

Acharya Balkrishna

^1,2,

Pradeep Nain

¹,

Monali Joshi

¹,

Lakshmipathi Khandrika

¹ and

Anurag Varshney

^1,2,*

¹Drug Discovery and Development Division, Patanjali Research Institute, NH-58, Haridwar 249 405, Uttarakhand, India

²Department of Allied and Applied Sciences, University of Patanjali, Patanjali Yog Peeth, Rorkee-Haridwar Road, Haridwar 249 405, Uttarakhand, India

Author to whom correspondence should be addressed.

Academic Editor: Eva Tvrdá

Molecules 2021, 26(4), 1020; https://doi.org/10.3390/molecules26041020

Received: 19 January 2021 / Revised: 6 February 2021 / Accepted: 9 February 2021 / Published: 15 February 2021

(This article belongs to the Special Issue Natural Bioactives: From Discovery to Applications in Reproduction)

Abstract

Putrajeevak (Putranjiva roxburghii Wall.; synonym Drypetes roxburghii (Wall.) Hurus) seeds have been used since ancient times in the treatment of infertility in the Ayurvedic system of medicine in India. In this study, the oil component of Putrajeevak seeds (PJSO) was extracted using the supercritical fluid extraction (SCFE) method using liquid CO₂ and the constituents were analyzed using gas chromatography-flame ionized detectorand high-performance thin-layer chromatography. PJSO contained trace amounts of β-sitosterol with oleic and linoleic acids as the major fatty acid constituents. Male and female zebrafish were mutagenized with N-ethyl-N-nitrosourea (ENU) and fish that produced less than 20 viable embryos were selected for the study. SCFE oil extracts from the P. roxburghii seeds were used in this study to reverse fertility impairment. The mutant fish were fed with PJSO for a period of 14 days and the rates of fertility, conception, and fecundity were determined with wild-type healthy fish as a breeding partner. Treatment with PJSO increased the ovarian follicle count as well as the number of mature eggs, while reducing the number of ovarian cysts. Sperm count as well as sperm motility were greatly enhanced in the ENU-mutagenized male zebrafish when treated with PJSO. The results obtained in this study demonstrate the effectiveness of P. roxburghii seed oil in reversing impaired fertility in both male and female zebrafish models. View Full-Text

Keywords: Putranjiva roxburghii; Putrajeevak seed oil; N-ethyl-N-nitrosourea mutagenesis; impaired fertility; zebrafish; β-sitosterol; follicle development; sperm motility